Hertta Pulkkinen

The Use of Recombinant Human Type II Collagen for Articular Cartilage Tissue Engineering

Opponent

Professor Johanna Myllyharju, University of Oulu, Finland

Reviewers

Professor Yrjö Konttinen, University of Helsinki, Finland

Professor Eero Vuorio, University of Turku, Finland

Supervisors

Professor Mikko Lammi, University of Eastern Finland, Finland

Professor Ilkka Kiviranta, University of Helsinki, Finland

Docent Virpi Tiitu, University of Eastern Finland, Finland

Articles of the thesis

Adult cartilage has a limited intrinsic capacity to regenerate and heal after injury. Therefore, a range of repair techniques have been adopted in an attempt to restore function and prevent further degeneration and development of osteoarthritis. Tissue engineering (TE) of cartilage using biodegradable material as a scaffold for chondrocytes could help the restoration of functional tissue. In this thesis a novel biomaterial, recombinant human type II collagen (rhCII), was tested as a scaffold for chondrocytes. The rhCII was proven to be a safe material for chondrocytes, and it allowed cell growth and production of cartilage ECM constituents, type II collagen and proteoglycans (PGs). When rhCII was used as a scaffold to repair osteochondral defects in a rabbit model, the repair tissue contained typical articular cartilage ECM and the use of rhCII helped to achieve better filling of the cartilage defect. The rhCII-assisted repair tissue integrated well to the subchondral bone and the tissue showed mechanical durability especially in the deeper zones. However, the repair quality was not much improved when compared to the spontaneous healing process. In addition, the superficial layer of repair tissue showed fibrillation and type I collagen production. Furthermore, the integration into the adjacent cartilage was not completein either rhCII assistedor the spontaneous repair tissue. Therefore, before rhCII can be used in human patients, further studies concerning enhancing the integration, and preventing surface fibrillation of the repair tissue, are needed.

  1. Pulkkinen H, Tiitu V, Lammentausta E, Laasanen MS, Hämäläinen ER, Kiviranta I, Lammi MJ: Cellulose sponge as a scaffold for cartilage tissue engineering. Bio-Med Mater Engin 16(4 Suppl):S29-S35, 2006 [Pubmed] [Full text]

  2. Pulkkinen HJ, Tiitu V, Valonen P, Hämäläinen ER, Lammi MJ, Kiviranta I: Recombinant human type II collagen as a material for cartilage tissue engineering. Int J Artif Organs 31(11):960-969, 2008 [Pubmed] [Full text]

  3. Pulkkinen HJ, Tiitu V, Valonen P, Jurvelin JS, Lammi MJ, Kiviranta I: Engineering of cartilage in recombinant human type II collagen gel in nude mouse model in vivo. Osteoarthritis Cartilage 18(8):1077-1087, 2010 [Pubmed] [Full text]

  4. Pulkkinen HJ, Tiitu V, Valonen P, Jurvelin JS, Rieppo L, Töyräs J, Silvast TS, Lammi MJ, Kiviranta I: Repair of osteochondral defects with recombinant human type II collagen gel and autologous chondrocytes in rabbit. Osteoarthritis Cartilage 21(3): 498-490, 2013 [Pubmed] [Full text]

Related articles

  1. Tiitu V, Pulkkinen HJ, Valonen P, Kiviranta O, Kiekara T, Kiviranta I, Lammi MJ: Recombinant human collagens as scaffold materials for chondrocyte cultures. Biomed Mater Eng 18(4-5):225-229, 2008 [Pubmed] [Full text]

  2. Tiitu V, Pulkkinen HJ, Valonen P, Pulliainen O, Kellomäki M, Lammi MJ, Kiviranta I: Bioreactor improves the growth and viability of chondrocytes in the knitted poly-L,D-lactide scaffold. Biorheology 45(3-4):539-546, 2008 [Pubmed] [Full text]

  3. Virén T, Saarakkala S, Jurvelin JS, Pulkkinen HJ, Tiitu V, Valonen P, Kiviranta I, Lammi MJ, Töyräs J: Quantitative evaluation of spontaneously and surgically repaired rabbit articular cartilage usingintra-articular ultrasound method in situ. Ultrasound Med Biol 36(5):833-839, 2010 [Pubmed] [Full text]

  4. Virén T, Huang YP, Saarakkala S, Pulkkinen H, Tiitu V, Linjama A, Kiviranta I, Lammi MJ, Brunott A, Brommer H, van Weeren R, Brama PAJ, Zheng YP, Jurvelin JS, Töyräs J: Comparison of ultrasound and optical coherence tomography techniques for evaluation of integrity of spontaneously repaired horse cartilage. J Med Eng Technol 36(3): 185-192, 2012 [Pubmed] [Full text]

Last updated January 29, 2019